Water analysis is the foundation of concern for people and living organisms. There are numerous microorganisms that can be dangerous to human health. As water is the most important substance on the planet for life, it is important to preserve it. Drinking water in the world is very small, and this small percentage is often polluted and is not healthy for use. Water is analyzed in several ways, and one of the most important is microbiological analysis. It was conducted on samples of water from shallow wells in the area of Virovitica-Podravina County. Water was analyzed by the most reliable method of microbiological analysis, which is the membrane filtration by which the bacteria are drawn to the nutrient base and grown. This raises the bacteria present in the water, and where some of them can be very dangerous to human health. The analysis shows that most of the water from shallow wells does not meet microbiological conditions and can thus endanger human health. Most bacteria in drinking water are coliform bacteria, and by this analysis numerous colonies of these bacterial species have been found.