The topic of this paper is harmful microorganisms that potentially disturb the microbiological purity in fast food facilities and procedures for preventing their growth and reproduction in places where they could create problems in the form of disease and infection. By not carrying out the necessary disinfection of the hands of employees who are in contact with the objects they use and by not carrying out good hygiene practices, possible infections develop. The aim of this study was to examine the microbiological cleanliness of surfaces and hands of employees in facilities to determine that they do not contain certain pathogenic microorganisms or that their number does not exceed the permissible limits. The samples examined were hands, accessories, equipment and other surfaces from ten different objects. After sampling with impression plates, the analysis was performed at the Public Health Institute of Požega-Slavonia County. The presence of Enterobacteriaceae and aerobic mesophilic bacteria was determined in microbiological analysis.