The aim of this work was to conduct microbiological and chemical analysis of chestnut honey. 5 samples were collected, 4 samples were from domestic producers, while 1 sample was from the retail chain. The analysis was carried out at the Institute of Public Health of Pozega - Slavonia County. The following chemical parameters were determined: the pH value of honey, the acidity of honey, the electroductivity of honey, the determination of the amount of water in honey and the pollen analysis of honey. After the completion of the chemical analysis according to the Honey Regulations (OG 53/15), no sample exceeds the permissible amount of water, while for electroductivity according to the same rulebook it does not meet one sample. According to the Honey Regulations (NN53/15), only one sample meets according to the permissible amount of free acids, while for pollen analysis according to the Uniflor honey Quality Regulations (OG122/09), two samples do not correspond. In microbiological analysis of the aerobic mesophilic bacteria Enterobacteriaceae, sulfitoreducing clostria and mold. After the analysis was carried out on all samples, no increase in any bacteria or mold was found.