Hygiene maintenance in food industries is of great importance for microbiological safety of final products and consumers health. The main objective of this final work is to question microbiological validity of water which is used for washing, cleaning and hygiene maintenance in a factory. Regarding, this final work will question the validity of factory hygiene and microbiological contamination of production line, machines and equipment, toghether with hygiene of employees in the factory for production of protein bars. The presence of microorganisms Pseudomonas aeruginosa, Clostridium perfringens, Escherichia coli, Enterococcus spp., total number of coliforms in sample of 100 mL and total number of microorganisms in sample of 1 mL at 36 °C and 22 °C using the membrane filtration method were determined. It has been found that tested samples are correct and within safety limits. During microbiological analysis of smear from machines, equipment, production line and hands of employees it has been determined the presence of microorganisms from the genus Enterobacteriaceae i Enterococcus spp. and total number of microorganisms in 1 mL of smear, ie lavage. Therefore, this analysis has proved microbiological safety of all tested samples.